10가지 종류의 상아질 지각과민처치제 세포독성 평가
Evaluation of cytotoxicity of 10 dentin desensitizers
손경미, 권지현, 박희철, 주정정, 임범순, 양형철,
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손경미 ( Son Kyung-Mi ) - 서울대학교 치의학대학원 치과생체재료과학교실
권지현 ( Kwon Ji-Hyung ) - 서울대학교 치의학대학원 치과생체재료과학교실
박희철 ( Park Hee-Chul ) - 서울대학교 치의학대학원 치과생체재료과학교실
주정정 ( Zhu Ting Ting ) - 서울대학교 치의학대학원 치과생체재료과학교실
임범순 ( Lim Bum-Soon ) - 서울대학교 치의학대학원 치과생체재료과학교실
양형철 ( Yang Hyeong-Cheol ) - 서울대학교 치의학대학원 치과생체재료과학교실
KMID : 0362220130400040263
Abstract
Dentin desensitizer is being widely used to treat hypersensitivity of teeth in dental clinics, and a variety of dentin desensitizers are commercially available. Because dentin desensitizers are applied close to oral tissues including gingiva and pulp, it is necessary to evaluate cytotoxicity of 10 desensitizers to secure the biological safety of the materials. In this study, we investigated cytotoxicity of dentin desensitizers by two different evaluation methods, mitochondrial dehydrogenase activity test with WST-8 and agar diffusion assay with neutral red. In the test with WST-8, Gluma Desensitizer, MicroPrime G, HurriSeal and Cervial Cement showed high cytotoxicity, followed by Zarosen, UltraEZ and Systemp desensitizer. In agar diffusion assay, Gluma Desensitizer, MicroPrime G and HurriSeal exhibited severe cytotoxicity, while other desensitizers showed moderate or mild cytotoxicity. Cervical Cement which exhibited relatively high toxicity in WST-8 assay showed mild cytotoxicity in the agar diffusion assay, of which difference was likely due to the difference of evaluation mechanism between WST-8 and neutral red. The highly cytotoxic Gluma Desensitizer, MicroPrime G, HurriSeal contain both or either of glutaraldehyde and 2-hydroxylethyl methacrylate (HEMA), suggesting that glutaraldehyde and HEMA induced strong cytotoxicity of the desensitizers.
키워드
Dentin desensitizer; cytotoxicity; mitochondrial dehydrogenase activity; WST-8; agar diffusion assay; neutral red
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